Klebsiella pneumoniae manipulates human macrophages to acquire iron
نویسندگان
چکیده
Background Klebsiella pneumoniae (KP) is a major cause of hospital-acquired infections, such as pneumonia. Moreover, it classified pathogen concern due to sprawling anti-microbial resistance. During infection, the gram-negative capable establishing an intracellular niche in macrophages by altering cellular metabolism. One factor critically affecting host-pathogen interaction availability essential nutrients, like iron, which required for KP proliferate but also modulates immune effector pathways. We hypothesized, that manipulates macrophage iron homeostasis acquire this crucial nutrient sustained proliferation. Methods applied in-vitro infection model, human macrophage-like PMA-differentiated THP1 cells were infected with (strain ATCC 43816). 24-h course we quantified number bacteria via serial plating cell lysates and evaluated effects different stimuli on bacterial numbers acquisition. Furthermore, analyzed host specific gene protein expression key metabolism molecules. Results Viable are recovered from during indicative persistence within inefficient clearing macrophages. Strikingly, following strongly induce main importer transferrin-receptor-1 (TFR1). Accordingly, proliferation further augmented addition loaded transferrin. The induction TFR1 mediated STAT-6-IL-10 axis, pharmacological inhibition pathway reduces uptake, elicits starvation, decreases survival. Conclusion Our results suggest, once confined inside enforces persistence. This facilitated microbial axis. Mechanistic insights into will provide opportunities development novel antimicrobial therapies.
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2023
ISSN: ['1664-302X']
DOI: https://doi.org/10.3389/fmicb.2023.1223113